pmRaspberry encodes mRaspberry, a mutant derived from the tetrameric Discosoma sp. red fluorescent protein, DsRed. In this vector, the mRaspberry coding sequence is flanked by MCS regions, making it easy to excise the gene for use in other cloning applications. pmRaspberry is primarily intended to serve as a source of mRaspberry cDNA.
Overview
- Far-red fluorescent protein ideal for in vivo applications
- mRaspberry fluorescent protein excitation and emission maxima: 598 and 625 nm, respectively
- Detect mRaspberry on a Western blot using these antibodies
Applications
- Fusions
- Protein localization studies
- In vivo imaging
Campbell, R. E. et al. A monomeric red fluorescent protein. Proc. Natl. Acad. Sci. U. S. A. 99, 7877–82 (2002).
Shaner, N. C. et al. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat. Biotechnol. 22, 1567–72 (2004).
Shu, X., Shaner, N. C., Yarbrough, C. A., Tsien, R. Y. & Remington, S. J. Novel chromophores and buried charges control color in mFruits. Biochemistry 45, 9639–9647 (2006).
Wang, L., Jackson, W. C., Steinbach, P. a & Tsien, R. Y. Evolution of new nonantibody proteins via iterative somatic hypermutation. Proc. Natl. Acad. Sci. U. S. A. 101, 16745–16749 (2004).
