pmR-ZsGreen1 is a mammalian expression vector designed to constitutively coexpress a microRNA (miRNA) of interest and the ZsGreen1 fluorescent protein. ZsGreen1 is a human codon-optimized variant of the Zoanthus sp. green fluorescent protein, ZsGreen, which has been engineered for brighter fluorescence and higher expression in mammalian cells. The unmodified vector will express ZsGreen1 in mammalian cells.
Overview
- Strong coexpression of miRNA and a bright fluorescent protein
- Clone your miRNA into the 3′ UTR of mCherry or ZsGreen1
- Simultaneously verify miRNA expression and transfection efficiency via fluorescent microscopy
Applications
- Over-expression of microRNAs to study gene or microRNA function
