This product is a plasmid vector for producing a high expression efficiency retrovirus vector. By transfecting this vector into an appropriate packaging cell line, the vector transiently or stably expresses transcribed products containing the virus-packaging signal (Ψ), target gene and selective marker. This vector possesses LTR and Ψ (viral packaging signal), but not the structural genes necessary for particle formation and replication (sequences gag, pol, and env). Because this vector contains an intron with high splicing capacity, it yields high transcription efficiency. pMEI-5 Neo DNA (Cat.# 3655) contains the neomycin resistance gene as a selective marker. Gene expression two to eight-fold higher than pDON-Al-2 series can be expected.
Overview
- Retroviral vectors designed for high retroviral titers and high expression of your transgene
- Compatible with all Takara Bio brand retroviral packaging cell lines
- Vector information is listed on our vector information pages
- Fluorescent protein, MSCV, and tetracycline-inducible retroviral vectors are described elsewhere
