This lentiviral expression vector encodes the ZsGreen1 fluorescent protein tag. The coding sequence for this very bright green fluorescent protein (2.5x brighter than EGFP) was modified to include human codon usage preferences, which allow improved expression in mammalian cells. Inserting a cDNA in the MCS downstream of the ZsGreen1 coding sequence joins your protein of interest to the C-terminus of the tag, and allows the fusion protein to be tracked and studied in transduced cells. To package the vector into high-titer, replication-incompetent lentivirus, we recommend using Lenti-X Packaging Single Shots and the Lenti-X 293T Cell Line. The resulting lentivirus can then be used to transduce virtually any mammalian cell type.
