This lentiviral expression vector encodes human β-actin fused to mCherry, an extremely bright red fluorescent protein. pLVX-mCherry-Actin constitutively expresses the mCherry-Actin fusion protein from PCMV IE when transduced into target cells. The mCherry-Actin fusion protein is incorporated into actin filaments, and allows for visualization of actin-containing subcellular structures in live cells as well as fixed cells. pLVX-mCherry-Actin is not designed to be used as a cloning vector.
To package the vector into high-titer, replication-incompetent lentiviral particles, we recommend using Lenti-X Packaging Single Shots (Cat. No. 632176) and the Lenti-X 293T Cell Line (Cat. No. 632180). The resulting lentivirus can then be used to transduce virtually any mammalian cell type.
