The pLVX-DsRed-Monomer-N1 vector is an HIV-1-based lentiviral expression vector that can produce recombinant lentivirus that efficiently transduces both dividing and non-dividing mammalian cells. This vector expresses a monomeric mutant of the Discosoma sp. red fluorescent protein (DsRed). pLVX-DsRed-Monomer-N1 allows cloning of a cDNA into the multiple cloning site (MCS) upstream of the DsRed-Monomer coding sequence. The cDNA is subsequently expressed as a fusion to the N-terminus of the DsRed-Monomer protein. Thus, this vector allows the expression of fluorescent fusion proteins in difficult-to-transfect cells. It also contains a puromycin selection marker for selection of stable transductants. The unmodified vector will express DsRed-Monomer protein and may be used to produce marker virus to optimize infection protocols.
