This lentiviral expression vector encodes an AmCyan1 fluorescent protein tag. The protein is a human codon-optimized variant of the Anemonia majano cyan fluorescent protein engineered for increased brightness and higher expression in mammalian cells. Inserting a cDNA in the MCS downstream of the AmCyan1 coding sequence joins your protein of interest to the C-terminus of AmCyan1, and allows it to be tracked and studied in transduced cells. To package the vector into high-titer, replication-incompetent lentivirus, we recommend using Lenti-X Packaging Single Shots (Cat. Nos. 631275–631278) and the Lenti-X 293T Cell Line (Cat. No. 632180). The resulting lentivirus can then be used to transduce virtually any mammalian cell type.
