PicoPLEX Single Cell WGA Kit v3
The PicoPLEX Single Cell WGA Kit v3 uses a single-tube protocol developed specifically for the reproducible amplification of genomic DNA (gDNA) starting from 1–10 cells or equivalent picogram quantities of isolated gDNA. Cell lysis and whole-genome preamplification are followed by high-fidelity amplification with very low background to yield over 2 µg of product in under 3 hours. This kit is suited for applications in which base-level resolution is required, such as for the detection of mutations.
Overview
- Reproducible results from single cells (or 6–50 pg of DNA)
- Superior reproducibility of allele representation
- Easy to use and automate
- Unambiguous results at all resolutions (e.g., microarray and PCR)
Applications
- CNV analysis using NGS, oligonucleotide aCGH, BAC aCGH, and qPCR
- Target enrichment or amplicon enrichment for mutation detection
- SNP genotyping
Suitable Inputs
- Mammalian cells (1–10 cells)
- Bacterial cells (1,000–10,000 cells)
- Isolated DNA (6–50 pg of human DNA)
- Sorted chromosomes
- Intact or fragmented, single- or double-stranded DNA
