The SMARTer Pico PCR cDNA Synthesis Kit provides a PCR-based method for producing high-quality cDNA from picogram quantities of total RNA. The cornerstone of SMARTer cDNA synthesis is still our unique SMART (Switching Mechanism At the 5′ End of RNA Transcript) technology; now with a modified SMARTer II A oligo. This new and improved oligo, combined with SMARTScribe Reverse Transcriptase, provides higher specificity and increased yields. The SMARTer Pico PCR cDNA Synthesis Kit allows you to synthesize high-quality cDNA for array probe generation, cDNA subtraction, “Virtual Northern” blots, or other applications, from as little as 1 ng of total RNA at extremely low concentrations (or in diluted samples). It is especially useful for researchers who have limited starting material, such as RNA derived from laser-capture microscopy samples, cells sorted by flow cytometry, or other extremely small samples.
Overview
- Start with as little as 1–2 ng of total RNA
- Specific enrichment for full-length cDNA
- Optimized protocol for retaining true gene representation
- Simplified protocol without DNase treatment or a separate adapter-ligation step
Applications
- Cloning and library construction
- Probe generation
- Subtractive hybridization
- Next-generation sequencing
- Virtual northerns
- Template generation for PCR/qPCR
