PhiX174 RF I DNA is a double-stranded circular DNA molecule composed of 5,386 base pairs. Restriction enzyme digest of phiX174 RF I DNA results in the generation of molecular weight size markers that can be used in nucleic acid gel analysis. PhiX174 double-stranded circular DNA is also frequently used in restriction enzyme quality control assays to assess endonuclease activity.
Bacteriophage phiX174 am3
> 70 double-stranded covalently closed circular form I (RF I)
–20°C
0.5 μg/μl
Air, G. M. et al. Nucleotide sequence of the F protein coding region of bacteriophage phiX174 and the amino acid sequence of its product. J. Mol. Biol. 125, 247–54 (1978).
Sanger, F. et al. Nucleotide sequence of bacteriophage phi X174 DNA. Nature 265, 687–95 (1977).
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