pHcRed1-N1/1 encodes HcRed1, a far red fluorescent protein that can be used to monitor gene expression and protein localization in vivo. HcRed1 was generated by mutagenesis of a non-fluorescent chromoprotein from the reef coral Heteractis crispa. The coding sequence for HcRed1 is human codon-optimized for higher expression in mammalian cells. Genes cloned into the multiple cloning site (MCS) upstream of the HcRed1 coding sequence are expressed as fusions to the N-terminus of HcRed1. The unmodified vector will express HcRed1 in mammalian cells.
Overview
- Far-red fluorescent protein, ideal for in vivo imaging
- HcRed1 fluorescent protein excitation and emission maxima: 588 and 618 nm, respectively
- HcRed1 is detected on a Western blot using anti-RCFP polyclonal pan antibody
Applications
- Fusions
- Protein localization studies
- General reporter for mammalian cells
- Monitoring transfection efficiencies
- In vivo imaging
