pHcRed1-Mito encodes a far-red fluorescent protein HcRed1 fused with mitochondrial targeting sequence from the precursor protein of human cytochrome c oxidase subunit VIII. HcRed1 is a fluorescent variant of a chromoprotein found in the reef coral Heteractis crispa. The coding sequence for HcRed1 has been human codon-optimized for higher expression in mammalian cells. pHcRed1-Mito can be used for specific labeling of mitochondria in living cells.
AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells.
Organelles targeted by the subcellular localization vectors.
Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.
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