pHcRed1-DR is a promoterless vector that encodes HcRed1-DR, a destabilized variant of Heteractis crispa far-red fluorescent protein, HcRed1. HcRed1-DR contains a PEST degradation domain at its C-terminus, so it is rapidly turned over in mammalian cell culture. Because if its short half-life, HcRed1-DR is well suited for monitoring the activity of promoters and promoter/enhancer combinations cloned into the multiple cloning site, located upstream of the human codon-optimized HCRed1-DR gene. Without the addition of a functional promoter, this vector will not express HcRed1-DR.
Overview
- Far-red fluorescent protein, ideal for in vivo imaging
- HcRed1 fluorescent protein excitation and emission maxima: 588 and 618 nm, respectively
- HcRed1 is detected on a Western blot using anti-RCFP polyclonal pan antibody
Applications
- Fusions
- Protein localization studies
- General reporter for mammalian cells
- Monitoring transfection efficiencies
- In vivo imaging
