pGBKT7 is a yeast expression vector that is designed to express a fusion protein of the GAL4 DNA-binding domain (DNA-BD; amino acids 1–147) and a bait protein. The fusion protein is expressed at high levels from the medium ADH1 promoter. The fusion protein also contains a c-Myc epitope tag. To facilitate in vitro transcription/translation, pGBKT7 includes the T7 promoter between the GAL4 DNA-BD and the epitope tag.
Overview
- The most popular and widely cited yeast two-hybrid vectors
- pGBKT7 and pGADT7 are components of the Matchmaker Gold Yeast Two-Hybrid System
- pBridge expresses a third protein for modified two-hybrid studies
Applications
- Yeast two-hybrid screening
- Yeast three-hybrid screening
