pEF1α-E2-Crimson is a mammalian expression vector that constitutively expresses the far-red fluorescent protein E2-Crimson, even after stable integration of the vector into the host cell genome. Stable, constitutive expression of E2-Crimson is driven by the human elongation factor 1 alpha (EF1α) promoter, which allows the protein to be expressed without the transgene silencing associated with CMV promoters. The vector, which lacks an MCS, is designed to be used for cell labeling or as a marker of transfection efficiency.
Overview
- Far-red fluorescent protein
- E2-Crimson fluorescent protein excitation and emission maxima: 611 and 646 nm, respectively
- Several of our red fluorescent protein antibodies strongly detect E2-Crimson
Applications
- Applications involving sensitive cells, such as primary cells and stem cells
- Fusions
- Protein localization studies
- General reporter for mammalian cells
- Monitoring transfection efficiencies
- In vivo imaging
