pE2-Crimson is a prokaryotic expression vector which encodes E2-Crimson, our brightest and fastest-maturing far-red Living Colors fluorescent protein. E2-Crimson was derived from DsRed-Express2, and retains its fast maturation, high photostability, increased solubility, and low cytotoxicity. pE2-Crimson is primarily intended to serve as a source of E2-Crimson cDNA. The flanking MCS regions make it possible to excise the E2-Crimson coding sequence and insert it into other expression vectors of choice. The vector can also be used in bacteria to produce E2-Crimson protein.
Overview
- Far-red fluorescent protein
- E2-Crimson fluorescent protein excitation and emission maxima: 611 and 646 nm, respectively
- Several of our red fluorescent protein antibodies strongly detect E2-Crimson
Applications
- Applications involving sensitive cells, such as primary cells and stem cells
- Fusions
- Protein localization studies
- General reporter for mammalian cells
- Monitoring transfection efficiencies
- In vivo imaging
