pE2-Crimson-C1 is a mammalian expression vector designed to express a protein of interest fused to the C-terminus of E2-Crimson, our brightest and fastest-maturing far-red Living Colors fluorescent protein. E2-Crimson was derived from DsRed-Express2, and retains its fast maturation, high photostability, increased solubility, and low cytotoxicity. The unmodified vector can be used to express E2-Crimson in mammalian cells. If required, stable transfectants can be selected using G418.
Overview
- Far-red fluorescent protein
- E2-Crimson fluorescent protein excitation and emission maxima: 611 and 646 nm, respectively
- Several of our red fluorescent protein antibodies strongly detect E2-Crimson
Applications
- Applications involving sensitive cells, such as primary cells and stem cells
- Fusions
- Protein localization studies
- General reporter for mammalian cells
- Monitoring transfection efficiencies
- In vivo imaging
