pDsRed2-Peroxi encodes a fusion of Discosoma sp. red fluorescent protein (DsRed2) and the peroxisomal targeting signal 1 (PTS1). The PTS1 sequence is fused to the 3′-end of DsRed2 and encodes the tripeptide SKL, which targets the fusion protein to the matrix of peroxisomes. DsRed2 is human codon-optimized for high expression in mammalian cells. pDsRed2-Peroxi is designed for fluorescent labeling of peroxisomes.
AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells.
Organelles targeted by the subcellular localization vectors.
Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.
Scientifix Pty Ltd
Unit F1, Hallmarc Business Park
2A Westall Road, Clayton, VIC 3168
Copyright © 2024 Scientifix
Site by Apex Strategy
