pDsRed2-Nuc encodes Discosoma sp. red fluorescent protein (DsRed2) fused with three copies of the nuclear localization signal (NLS) of the SV40 T-antigen. The NLS sequences are fused to the 3′-end of DsRed2. DsRed2 is a human codon-optimized variant of wild-type DsRed that has been engineered for faster maturation and lower non-specific aggregation. pDsRed2-Nuc is designed for fluorescent labeling of the nucleus in living cells.
AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells.
Organelles targeted by the subcellular localization vectors.
Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.
Scientifix Pty Ltd
Unit F1, Hallmarc Business Park
2A Westall Road, Clayton, VIC 3168
Copyright © 2024 Scientifix
Site by Apex Strategy
