pDsRed-Monomer-N In-Fusion Ready Vector encodes DsRed-Monomer, a monomeric mutant of the Discosoma sp. red fluorescent protein. The vector is prelinearized to enable directional In-Fusion cloning of PCR fragments onto the 5′ end of the DsRed-Monomer-encoding gene without further restriction digests. Sequences complementary to the vector sequences (flanking the site of linearization) need to be incorporated at the 5′ ends of the PCR primers (see PT3865-2). The gene of interest is expressed as an N-terminal fusion to DsRed-Monomer in mammalian cells. The fluorescent protein coding sequence in this construct has been human codon-optimized for efficient expression and enhanced brightness in mammalian cells.
