pDsRed-Express2 is a prokaryotic expression vector that encodes DsRed-Express2, a variant of Discosoma sp. red fluorescent protein. pDsRed-Express 2 is primarily intended to serve as a source of DsRed-Express 2 cDNA. The flanking MCS regions make it possible to excise the DsRed-Express2 coding sequence and insert it into other expression vectors of choice. The vector can also be used in bacteria to produce DsRed-Express2 protein.
Robust expression of DsRed-Express and DsRed-Express2 in mouse bone marrow hematopoietic stem and progenitor cells. Mononuclear bone marrow cells were transduced with retroviral vectors encoding DsRed-Express (Panel A), DsRed-Express2 (Panel B), or EGFP (Panel C), and fluorescent cells were sorted 87 hr later. Red and green fluorescence signals were detected using the PE and FITC filter sets, respectively. The lines represent gates defined by analyzing untransduced cells.
Basic fluorescent protein vector map. Use this bacterial expression vector as a source of the fluorescent protein gene. Note: There is a stop codon at the 5′ end of the 3′ MCS. The 3′ MCS should not be used for cloning.
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