The mammalian CMV promoter in pCMV-tdTomato allows the constitutive expression of the tandem version of the dimeric red fluorescent protein, Tomato, driven by the constitutive immediate early promoter of cytomegalovirus (CMV). This vector does not contain an MCS. It can be used for labeling purposes or as a cotransfection marker to determine transfection efficiency.
tdTomato, but not GFP, can be detected in the SCID mouse cadaver phantom model. False-color overlay images (regions of interest encircled) indicate that the imaging system could detect tdTomato fluorescence in the cadaver model, but not GFP fluorescence. Panel A. Implanted tube with 100 x 106 MDA-MB-231-tdTomato-expressing cells, imaged with the DsRed filter set. Exposure time: 1 sec. Panel B. Implanted tube with 100 x 106 MDA-MB-231-GFP-expressing cells, imaged with the GFP filter set. Exposure time: 1 sec.
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