pCMV DsRed-Express2 is designed for whole cell labeling or to be used as a cotransfection marker in mammalian cells; the red fluorescence of DsRed-Express2 can be detected by fluorescence microscopy or flow cytometry, providing direct visual evidence of transfection or the ability to monitor cells after transplantation experiments in vivo. After cotransfection with pCMV DsRed-Express2 and an expression construct of interest, cells can also be sorted by flow cytometry (FACS) to enrich for transfected cells. pCMV DsRed-Express2 Vector can be transfected into mammalian cells using any standard transfection method. If required, stable transfectants can be selected using G418.
