The pBI bidirectional vectors are designed to constitutively express a protein of interest and a reporter protein (or two proteins of interest). Expression is driven by constitutively active bidirectional human cytomegalovirus promoters (PminCMV). The gene(s) of interest must contain an initiation codon and a stop codon.
Overview
- Constitutively express a protein of interest and a reporter protein (or two proteins of interest)
- Choose from green or red fluorescent reporters
- pBI-CMV1 has two cloning sites and allows the constitutive expression of two proteins of interest.
- pBI-CMV2 allows you to express a protein of interest and AcGFP1, a monomeric green fluorescent protein derived from Aequorea coerulescens.
- pBI-CMV3 allows you to express a protein of interest and ZsGreen1, an extremely bright green fluorescent protein derived from Zoanthus sp. reef coral.
- pBI-CMV4 allows you to express a protein of interest and DsRed2, a red fluorescent protein derived from Discosoma sp. reef coral.
- pBI-CMV5 allows you to express a protein of interest and firefly luciferase. pBI-CMV5 is not sold separately. It is sold as part of the Bidirectional Secreted Luciferase System, together with its reporter assay.
