The pAutophagSENSE vector expresses the mutant green fluorescent protein AcGFP1 (excitation maximum = 475 nm; emission maximum = 505 nm) fused to the mouse LC3 protein in mammalian cells. The fusion protein is incorporated into the autophagosomes and is used to monitor autophagosome formation in live cells. This process is monitored by following the redistribution of a green fluorescent fusion protein from the cytosol to the forming autophagosomes.
