This vector is designed for monitoring the activation of the activation of activator protein 1 (AP1) by phorbol esters (PMA). The AP1(PMA) element responds specifically to PMA treatment for studying activation of the protein kinase C (PKC) pathway. When transfected into mammalian cells, the vector can be used for studying effects of activation of this and related signal transduction pathways. The vector contains the gene encoding firefly luciferase as the reporter, which can be measured by any standard luciferase assay.
Overview
- Extensive coverage of cell signaling pathways
- Easy to use & reliable
