The Pantropic Retroviral Expression System is a complete retroviral-mediated gene expression system designed to infect dividing cells from a broad range of mammalian and nonmammalian cell types. The included GP2-293 Packaging Cell Line contains only the viral gag and pol genes. The viral envelope is supplied by transiently transfecting with pVSV-G, which is also included.
Overview
- We recommend the Retro-X Universal Packaging System for most applications
- This system produces the highest titers
- GP2-293 cells are easy to transfect which leads to very high titers
- It is supplied with all four commonly used envelopes for great flexibility (including VSV-G and Eco)
- Retrovirus is produced by transient transfection of the envelope vector and retrovirus vector
- Use RetroPack cells if you wish to create a stable producer cell line
- All retroviral packaging cell lines and systems are compatible with all retroviral vectors sold by Takara Bio, including all MMLV- and MSCV-based retroviruses.
