pAmCyan1-N1 is a human codon-optimized expression vector that encodes a variant of the Anemonia majano cyan fluorescent protein, AmCyan1, which has been engineered for brighter fluorescence and higher expression in mammalian cells. pAmCyan1-N1 allows genes cloned into the multiple cloning site (MCS) upstream of the AmCyan1 coding sequence to be expressed as fusions to the N-terminus of the fluorescent protein. The unmodified vector will express AmCyan1 in mammalian cells.
Schematic for fluorescent fusion protein vectors. Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector.
Hygromycin DsRed-Monomer and AcGFP1 expression vectors. Vector maps of our DsRed-Monomer and pAcGFP1 Hyg-N1 (Panel A) and Hyg-C1 (Panel B) mammalian expression vectors for fusion applications. Each vector contains a hygromycin selection cassette. These vectors can be used together with our standard N1/C1 vectors containing the neomycin selection cassette to establish double stable cell lines expressing two different Living Colors proteins.
Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1.
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