pAmCyan1-C1 is a human codon-optimized expression vector that encodes a variant of the Anemonia majano cyan fluorescent protein, AmCyan1, which has been engineered for brighter fluorescence and higher expression in mammalian cells. pAmCyan1-C1 allows genes cloned into the multiple cloning site (MCS) downstream of the AmCyan1 coding sequence to be expressed as fusions to the C-terminus of the fluorescent protein. The unmodified vector will express AmCyan1 in mammalian cells.
Schematic for fluorescent fusion protein vectors. Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector.
Hygromycin DsRed-Monomer and AcGFP1 expression vectors. Vector maps of our DsRed-Monomer and pAcGFP1 Hyg-N1 (Panel A) and Hyg-C1 (Panel B) mammalian expression vectors for fusion applications. Each vector contains a hygromycin selection cassette. These vectors can be used together with our standard N1/C1 vectors containing the neomycin selection cassette to establish double stable cell lines expressing two different Living Colors proteins.
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