pAmCyan is a prokaryotic expression vector, which encodes Anemonia majano cyan fluorescent protein (AmCyan). The AmCyan coding sequence carried in pAmCyan retains the wild-type codon usage. In this vector, the mCyan gene is flanked by separate and distinct MCS regions at the 5′ and 3′ ends so that the AmCyan gene can be subcloned into other expression vectors. This vector is primarily intended to serve as a convenient source of AmCyan cDNA.
Basic fluorescent protein vector map. Use this bacterial expression vector as a source of the fluorescent protein gene. Note: There is a stop codon at the 5′ end of the 3′ MCS. The 3′ MCS should not be used for cloning.
Four-color visualization of Living Colors Fluorescent Proteins. HEK 293 clonal cell lines stably expressing AmCyan1, ZsGreen1, ZsYellow1, or HcRed1 were mixed and plated in the same culture dish and imaged at 20X magnification.
Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1.
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