pAcGFP1-1 encodes a green fluorescent protein (AcGFP1) from Aequorea coerulescens. The fluorescent protein coding sequence in this construct has been human-codon-optimized for efficient expression and enhanced brightness in mammalian cells. The vector contains a neomycin resistance gene for the selection of stable transfectants. pAcGFP1-1 can be used as a reporter to monitor the activity of promoters and promoter/enhancer combinations cloned into the multiple cloning site (MCS) upstream of the promoterless AcGFP1 gene. Without the addition of a functional promoter, this vector will not express AcGFP1.
