The Vectalys Constitutive Lentiviral Reporter Vectors are prepackaged lentiviral particles expressing fluorescent protein genes for transducing mammalian cells. The expression of fluorescent protein genes is driven by an EF1α promoter. These VSV-G pseudotyped viruses are capable of infecting both dividing and non-dividing cells. The high purification level of these lentiviral particles allows transduction of primary cells and immortalized cell lines without inducing any toxicity to the cells, even at high multiplicity of infection (M.O.I.).
Overview
- What does 109 TU/ml mean?
- In each microliter, there will be at least 1 x 106 functional lentivirus particles.
- Each 60-µl kit is sufficient for 30 transductions (using an MOI of 10) in a 6-well plate containing 2 x 105 cells/well.
- Ready-to-use particles can be added directly to cells:
- Highly concentrated lentiviral particles; all lots are >109 transducing units (TU) per ml (functional titer, not a just a particle count).
- High-purity lentiviral particles—transduce cells without cytotoxicity.
- Expression can be monitored in living cells by fluorescence microscopy
- AcGFP1 is a true monomeric green fluorescent protein with spectral properties similar to those of EGFP.
- mCherry is the most widely adopted monomeric red fluorescent protein.
- Learn more about the properties of these fluorescent proteins.
- Particles are available for labeling the nucleus, endoplasmic reticulum, actin, mitochondria, tubulin, endosome, golgi, and plasma membrane.
- These vectors have a puromycin resistance cassette and the stable clones integrating these constructs can be selected using puromycin.
Applications
- Cell organelle/structure labeling—monitor protein localization, protein trafficking, organelle dynamics, etc.
- Simple method for generating stable reporter cell lines
