Applications
- Poly(A) mRNA isolation from total RNA
- Clean up of in-vitro transcripts
- Direct purification of poly(A) mRNA from cells
Features
- Convenient processing by microcentrifugation using NucleoTrap® Microfilters
- Direct mRNA isolation from cells
- High quality poly(A) mRNA without degradation and DNA contamination
Specifications
- Technology: Affinity chromatography
- Format: Oligo(dT) latex bead suspension
- Fragment size: 50 nt–20 knt
- A260/A280: 1.9–2.1
- Elution volume: 10–20 μL
- Binding capacity: 0.25 μg poly(A) mRNA/μL oligo(dT) latex bead suspension
- Processing time: 30 min/6 preps
NucleoTrap® mRNA Mini
- Sample material: < 250 μg total RNA
- Typical yield: 10 μg mRNA
NucleoTrap® mRNA Midi
- Sample material: < 1000 μg total RNA
- Typical yield: 40 μg mRNA
