Applications
- RNA isolation from cultured cells, tissue (standard protocol)
- RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells
- RNA from ≤ 100 μL biological fluids
- RNA clean-up from reaction mixtures
- RNA from samples stored in RNAlater®
- RNA from saliva samples collected with Oragene®•RNA (Genotek)
- Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays
Features
- rDNase included for on-column DNA digestion– easy and efficient removal of contaminating DNA
- NucleoSpin® Filters (shredders) – efficient sample homogenization and reduction of viscosity
- Up to 70 μg ready to use RNA
- Parallel purification of genomic DNA by using the NucleoSpin® RNA/DNA Buffer Set
Specifications
- Technology: Silica membrane technology
- Fragment size: > 200 nt
- A260/A280: 1.9–2.1
- Processing: Centrifugation
- Sample material: Cultured cells (< 5 x 10⁶) bacterial cells (< 10⁹), yeast cells (< 10⁸) Human / animal tissue (< 30 mg)
- Typical yield: HeLa cells (10⁶): 14 μg, bacterial cells (10⁹): 70 μg
- Elution volume: 30–120 μL
- Binding capacity: 200 μg
- Processing time: 35 min/6 preps
