Applications
- RNA isolation from cultured cells, animal tissue, plant tissue, microorganisms
- Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays
Features
- NucleoSpin® gDNA Removal Column – no time consuming rDNase digestion
- Convenient handling – lysate clearing and gDNA removal with one column in one step
- New efficient lysis buffer – no b-mercaptoethanol or TCEP necessary
Specifications
- Technology: Silica membrane technology (1. column for DNA removal and lysate clearing, 2. column for RNA isolation)
- A260/A280: 1.9–2.1
- Sample material: Cultured cells (< 10⁷), bacterial cells (< 10⁹), yeast cells (< 10⁸), human / animal tissue (< 30 mg)
- Fragment size: > 200 nt
- Typical yield: 40–100 μg
- Elution volume: 30–120 μL
- Binding capacity: 200 μg
- Processing time: 20 min/6 preps
