NucleoSpin Plasmid / Plasmid (No Lid)

NucleoSpin Plasmid / Plasmid (No Lid)

Additional information

Brand	MACHEREY-NAGEL

Applications

High- and low‑copy* plasmid DNA purification from E. coli cultures and Gram positive bacteria
Plasmid DNA clean up from reaction mixtures

Features

High capacity – up to 50 μg of plasmid DNA
Flexible solutions for full automation available
Optional washing step for highest plasmid DNA quality

Specifications

Technology: Silica membrane technology
Endotoxin level: >> 50 EU/μg DNA
Processing: Manual (centrifugation or vacuum)
Sample material: 2–5 mL (high‑copy plasmid), 5–10 mL (low‑copy plasmid)
Vector size: < 25 kbp
Typical yield: 25–45 μg
Elution volume: 50 μL
Binding capacity: 50 μg
Processing time: 25 min/6 preps

High yield plasmid Mini prep

Plasmid DNA isolation (pUC18) from E. coli DH5α using NucleoSpin® Plasmid. For the isolation 2 mL (lane 1–2), 5 mL (lane 3–4) and 8 mL (lane 5–6) LB cultures were used and analyzed on an agarose gel (2 μL of each eluate). Lane M: marker.

Plasmid DNA Purification Guide Brochure 740588.10 NucleoSpin Plasmid SDS 740588.50 NucleoSpin Plasmid SDS 740588.250 NucleoSpin Plasmid SDS 740499.10 NucleoSpin Plasmid (No Lid) SDS 740499.50 NucleoSpin Plasmid (No Lid) SDS 740499.250 NucleoSpin Plasmid (No Lid) SDS NucleoSpin Plasmid Instruction