Applications
- Manual and automated isolation of high-copy plasmids
- Typical downstream applications: transfection of common cell lines, cell-based assays, screenings
Features
- Novel technology to diminish endotoxin content (< 50 EU/µg DNA) for successful transfections
- Innovative MN Wash Plate minimizes risk of cross-contamination
- NucleoSpin® 96 Plasmid Transfection-grade Core Kits*: Kits with basic content focusing on automation platforms. Additional accessories can be combined as needed.
Specifications
- Technology: Silica-membrane and endotoxin-reduction technology
- Format: 96-well plate
- Processing: Manual or automated, vacuum or centrifugation
- Endotoxin content: < 50 EU/µg DNA (EU=Endotoxin Units)
- Sample material: Up to 5 mL bacterial culture (E. coli, high-copy plasmids)
- Vector size: < 15 kbp
- Typical yield: 5–20 µg
- A260/A280: 1.80–1.85
- Elution volume: 100–200 µL
- Preparation time: 45 min/plate
- Binding capacity: 20 µg


