Applications
- High- and low‑copy plasmid DNA purification from E. coli cultures
Features
- Cost-efficient solution for plasmid DNA isolation
- Protocol for large, low‑copy constructs available
Specifications
- Technology: Alkaline lysis with subsequent filtration and precipitation
- Endotoxin level: >> 50 EU/μg DNA
- Processing: Manual or automated
- Sample material: 1.1–1.3 mL (high‑copy plasmid), 1.1–3.9 mL (low‑copy plasmid)
- Vector size: < 250 kbp
- Typical yield: 8 μg (1.3 mL high‑copy plasmid), 1 μg (1.3 mL low‑copy plasmid)
- Processing time: 90 min/184 preps
