NucleoSpin 8 / 96 Plasmid Kits and Core Kits

High yield plasmid Mini prep

Plasmid DNA isolation (pUC18) from E. coli DH5α using NucleoSpin® Plasmid. For the isolation 2 mL (lane 1–2), 5 mL (lane 3–4) and 8 mL (lane 5–6) LB cultures were used and analyzed on an agarose gel (2 μL of each eluate). Lane M: marker.

Isolation of plasmid DNA from bacterial cultures on Hamilton [MPE]²

Plasmid DNA of two different bacterial strains, transformed with plasmids containing either a 1482 bp or a 982 bp inserts, was isolated from 1.5 mL of bacterial cultures (E. coli DH 5α™, high-copy plasmid pGEM®-T Easy; n=24) using the NucleoSpin® 96 Plasmid kit on a [MPE]² positive pressure module (dark blue) or a manual vacuum manifold (light blue). Total yield was determined by UV spectrometry showing comparable yields between positive pressure or vacuum processed samples.

Purity of isolated plasmid DNA from bacterial cultures

Plasmid DNA of two different bacterial strains, transformed with plasmids containing either a 1482 bp or a 982 bp inserts, was isolated from 1.5 mL of bacterial cultures (E. coli DH 5α, high copy plasmid pGEM®-T Easy; n=24) using the NucleoSpin® 96 Plasmid kit on a [MPE]² positive pressure module (A260/A280: dark blue bars; A260/A230: orange squares) or a manual vacuum manifold (A260/A280: light blue bars; A260/A230: orange squares). Purity was determined by UV spectrometry revealing comparable quality of positive pres‑ sure or vacuum processed samples.