NucleoSnap Plasmid Midi

Additional information

Brand

Applications

  • High- and low‑copy plasmid DNA purification from E. coli cultures

Features

  • New column design (snap off column) for vacuum processing of large sample volumes
  • Isolate up to 700 μg plasmid DNA in only 35 minutes
  • No need for time consuming DNA precipitation

Specifications

  • Technology: Precipitation and filtration
  • Processing: Vacuum processing, centrifugation for elution
  • Endotoxin level: < 50 EU/μg DNA
  • Sample material: ≤ 50 mL
  • Vector size: < 25 kbp
  • Typical yield: 400–700 μg
  • Elution volume: 200–500 μL
  • Binding capacity: 1.5 mg
  • Processing time: 35 min/6 preps

Superior performance in electroporation experiment

Eukaryotic cells were manipulated with the Nucleofector™ technology. The viability of cells treated with plasmid DNA isolated with the NucleoSnap® Plasmid Midi kit is comparable to the viability of cells in the mock control (grey bar), indicating that the plasmid DNA does not affect cell viability. In contrast, Nucleofection™ with plasmid DNA isolated with the molecular biology-grade NucleoSpin® Plasmid kit leads to a decrease in cell viability.

Procedure

NucleoSnap Plasmid Midi Flyer