Applications
- High- and low‑copy* plasmid DNA purification from E. coli cultures
Features
- Column filter for fast and easy lysate clarification included – high filter flow rates, parallel lysate clearing and loading onto the column
- Midi and Maxi preps with extremely high yield
- NucleoSpin® Finishers, NucleoSnap® Finishers, and NucleoBond® Finalizers are available as separate tools to avoid time consuming centrifugation for plasmid precipitation
Specifications
- Technology: Anion exchange chromatography, gravity flow columns
- Endotoxin level: 1–10 EU/μg DNA
- Sample material: < 200 mL (high‑copy plasmid), < 400 mL (low‑copy plasmid)
- Typical yield: 400 μg
- Processing time: 70 min/prep (NucleoBond® Xtra Midi), 30 min/prep (NucleoBond® Xtra Midi Plus, incl. NucleoBond® Finalizer)
