Applications
- RNA isolation from soil for qRT-PCR analysis
- Typical downstream applications: real-time RT-PCR
Features
- Anion exchange technology in Mini format to optimize RNA yield and purity – suitable for qRT-PCR
- Combination of mechanical homogenization and chemical lysis allows processing of large sample amounts
- Parallel preparation of RNA and DNA in one hour
Specifications
- Technology: Anion exchange chromatography, gravity flow columns combined with NucleoSpin® Bead Tubes Type A
- Format: Gravity flow columns
- Sample material: 0.25–0.5 g soil
- Fragment size: > 100 nt
- Typical yield: 0.25–2.5 μg
- A260/A280: 1.5–2.0
- Typical RIN (RNA integrity number): > 7.0
- Elution volume: 50–100 µL
- Preparation time: 60 min/12 preps
- Binding capacity: 30 µg
