Applications
- High- and low‑copy* plasmid DNA purification from E. coli cultures
Features
- Clarification of lysates with NucleoBond® Folded Filters, no centrifugation required, no shearing forces
- NucleoSpin® Finishers, NucleoSnap® Finishers, and NucleoBond®
- Finalizers are available as separate tools to avoid time consuming centrifugation for plasmid precipitation (see pages 29, 30, 31)
- Separate kit components available: NucleoBond® AX Columns, RNase, and buffers
Specifications
- Technology: Anion exchange chromatography, gravity flow columns
- Endotoxin level: 1–10 EU/μg DNA
NucleoBond® PC 20
- Sample material: 1–5 mL (high‑copy plasmid), 3–10 mL (low‑copy plasmid)
- Typical yield: 3–20 μg
- Binding capacity: 20 μg
NucleoBond® PC 100
- Sample material: 5–30 mL (high‑copy plasmid), 10–100 mL (low‑copy plasmid)
- Typical yield: 20–100 μg
- Binding capacity: 100 μg
NucleoBond® PC 500
- Sample material: 30–150 mL (high‑copy plasmid), 100–500 mL (low‑copy plasmid)
- Typical yield: 400–500 μg
- Binding capacity: 500 μg
NucleoBond® PC 2000
- Sample material: 150–500 mL (high‑copy plasmid), 500–2000 mL (low‑copy plasmid)
- Typical yield: 500–2000 μg
- Binding capacity: 2000 μg
NucleoBond® PC 10000
- Sample material: 500–2000 mL (high‑copy plasmid), 1–4 L (low‑copy plasmid)
- Typical yield: 2000–10000 μg
