NheI

Brand	Takara Bio

NheI restriction site:

G | CTAG C
C GATC | G

Supplied buffer: M

Effect of DNA methylation: enzyme activity is affected by CG methylase

Reaction temperature: 37°C

Source: Neisseria mucosa heidelbergensis
Concentration: 4–12 units/µl
Substrate for unit definition: lambda DNA
Genome DNA analysis: Arthrobacter luteus genome DNA
Star activity: unrelated site may be cut in the presence of DMSO, high concentrations of glycerol, alkaline pH, or low ionic strength
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning