NcoI

Brand	Takara Bio

NcoI restriction site:

C | CATG G
G GTAC | C

Supplied buffer: K+BSA

Reaction temperature: 37°C

Source: Nocardia coralline
Concentration: 4–12 units/µl [High concentration: 30–60 units/µl]
Substrate for unit definition: lambda DNA
Star activity: unrelated site may be cut in the presence of DMSO or high concentrations of glycerol
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning