NaeI

Brand	Takara Bio

NaeI restriction site:

GCC | GGC
CGG | CCG

Supplied buffer: L

Effect of DNA methylation: enzyme activity is affected by CG methylase

Reaction temperature: 37°C

Source: Nocardia aerocolonigenes
Concentration: 4–12 units/µl
Substrate for unit definition: pBR322 DNA
Genome DNA analysis: Staphylococcus aureus genome DNA
Notice: this enzyme is able to cleave at 4 pBR322 sites, but the site at position 1283 is resistant to complete cleavage
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning