Applications
- RNA isolation from cultured cells
- RNA isolation from tissue
- RNA isolation from cryosections
- RNA isolation from laser captured cells
- RNA isolation from small amounts of plant material
- RNA isolation from samples stored in RNAlater®
- Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays
Features
- Isolation of RNA from small sample quantities like biopsy material or single cells
- Excellent RNA recovery and integrity
- Concentrated RNA for sensitive downstream applications by elution in as little as 5 μL
- rDNase included for on-column DNA removal
- NucleoSpin® Filters (shredders) – efficient homogenization and reduction of viscosity
- High quality RNA, ready to use for RT-PCR and other applications
- Reducing agent TCEP – no ß-mercaptoethanol necessary
Specifications
- Technology: Silica membrane technology
- Fragment size: > 200 nt
- A260/A280: 1.9–2.1
- Processing: Centrifugation
- Sample material: Cultured cells (1–10⁵), human / animal tissue (< 5 mg)
- Typical yield: HeLa cells (10²): 0.1–1.5 ng, HeLa cells (10⁵): 1–1.5 μg
- Elution volume: 5–30 μL
- Binding capacity: 110 μg
- Processing time: 35 min/6 preps
