Applications
- RNA isolation from small amounts of cultured cells, human / animal tissue
- RNA from samples stored in RNAlater
- Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays, RNA-sequencing
Features
- Developed for extra small samples down to single cell analysis
- gDNA removal column to omit time consuming DNA digestion
- Novel lysis buffer without mercapthoethanol or TCEP
Specifications
- Technology: Silica membrane technology (1. column for DNA removal and lysate clearing, 2. column for RNA isolation)
- A260/A280: 1.9–2.1
- Sample material: Cultured cells (1–10⁵), human / animal tissue (< 5 mg)
- Fragment size: > 100 nt
- Typical yield: HeLa cells (10¹): 0.05–0.02 ng, HeLa cells (10⁵): 0.5–2.0 μg, mouse liver (0,5 μg): 2.5–8 ng, mouse brain (0.5 μg): 0.1–0.5 ng
- Elution volume: 5–30 μL
- Binding capacity: 110 μg
- Processing time: 18 min/6 preps


