MboII

Brand	Takara Bio

MboII restriction site:

GAAGA(N)8 |

CTTCT(N)7 |

Supplied buffer: L

Effect of DNA methylation: enzyme activity is affected by dam methylase

Reaction temperature: 37°C

Source: Moraxella bovis
Concentration: 4–12 units/µl
Substrate for unit definition: lambda DNA
Ligation-recutting test: The ligation efficiency of cohesive ended DNA fragments generated by this enzyme is low. More efficient ligation can be achieved using blunt end ligation reaction conditions.
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning