This yeast two-hybrid library was constructed from mRNA isolated from 11 Arabidopsis tissues, mixed in equal quantities and transformed into yeast strain Y187. The cDNA was normalized prior to library construction to reduce the copy number of abundant cDNAs derived from highly represented mRNAs, thereby increasing the representation of low copy number transcripts. The normalization process combines a Duplex-Specific Nuclease (DSN) treatment and SMART technology, reduces the number of clones that must be screened in your yeast two-hybrid assay, and facilitates the identification and characterization of novel protein-protein interactions. The library was transformed into yeast strain Y187 and can be readily mated to a MATa GAL4 reporter strain, such as AH109 or Y2HGold, for screening.
Overview
- By far the easiest way to screen a library for protein-protein interactions
- Screen fewer colonies, detect more interactions
- Universal libraries—broadest gene representation
- No more searching for needles in a haystack
